γ, δ, ε, and ζ Tubulin Mutations

Gamma Tubulin Mutations

XRD AA1 AA3 Tubulin Gene (Isotype) Organism Phenotype Reference Keywords Notes
R3A Arg3Ala CAA33507.1 A. nidulans With E4A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R3A Arg3Ala CAA19365.1 S. pombe With E4A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S1 E4A Glu4Ala CAA33507.1 A. nidulans With R3A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S1 E4A Glu4Ala CAA19365.1 S. pombe With R3A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S1 Q9L Gln9Leu NP_598789.1 (Tubg2) M. musculus IGL02302:Tubg2, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
H1 Q12G Gln12Gly AAB67442.1 (Tub4p) S. cerevisiae With C13G and T146A, GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
H1 C13A Cys13Ala AAB65830.1 T. thermophila Weak cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H1 C13G Cys13Gly AAB67442.1 (Tub4p) S. cerevisiae With Q12G and T146A, GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
H1 C13S Cys13Ser AAB67442.1 (Tub4p) S. cerevisiae With D70N, GTP binding; with T146A, GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
H1 N15K Asn15Lys NP_598785.1 (Tubg1) M. musculus R5271:Tubg1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
H1 E20A Glu20Ala CAA19365.1 S. pombe With E20A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H1 K23A Lys23Ala CAA19365.1 S. pombe With K23A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H1 Q24V Gln24Val AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H1 E28A Glu28Ala CAA33507.1 A. nidulans Wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H1 H29A His29Ala CAA33507.1 A. nidulans Wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
E38A Glu38Ala CAA33507.1 A. nidulans With E39A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
E38A Glu38Ala CAA19365.1 S. pombe With E39A and E43A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
E39A Glu39Ala CAA33507.1 A. nidulans With E38A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
E39A Glu39Ala CAA19365.1 S. pombe With E38A and E39A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
E43A Glu43Ala CAA33507.1 A. nidulans With D46A and R47A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
E43A Glu43Ala CAA19365.1 S. pombe With E38A and E39A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D46A Asp46Ala CAA33507.1 A. nidulans With E43A and R47A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
D46A Asp46Ala CAA19365.1 S. pombe With R47A, K48A, D49A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
R47A Arg47Ala CAA33507.1 A. nidulans With E43A and D46A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R47A Arg47Ala CAA19365.1 S. pombe With D46A, K48A, D49A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K48A Lys48Ala CAA33507.1 A. nidulans With D49A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
K48A Lys48Ala CAA19365.1 S. pombe With D46A, R47A, D49A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K48A Lys48Ala AAB65830.1 T. thermophila With E49A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
D49A Asp49Ala CAA33507.1 A. nidulans With K48A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
D49A Asp49Ala CAA19365.1 S. pombe With D46A, R47A, K48A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
E49A Glu49Ala AAB65830.1 T. thermophila With K48A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S4 D56A Asp56Ala CAA33507.1 A. nidulans With D57A and R59A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S4 D56A Asp56Ala CAA19365.1 S. pombe With D57A and E58A, dominant cold sensitive as diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S4 D56A Asp56Ala AAB65830.1 T. thermophila With D57A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
D57A Asp57Ala CAA33507.1 A. nidulans With D56A and R59A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
D57A Asp57Ala CAA19365.1 S. pombe With D56A and E58A, dominant cold sensitive as diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D57A Asp57Ala AAB65830.1 T. thermophila With D56A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S5 E58A Glu58Ala CAA19365.1 S. pombe With D56A and D57A, dominant cold sensitive as diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S5 H59A His59Ala AAB65830.1 T. thermophila With R63A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S5 R59A Arg59Ala CAA33507.1 A. nidulans With D56A and D57A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S5 R63A Arg63Ala CAA33507.1 A. nidulans Cold sensitive and heat sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S5 R63A Arg63Ala AAB65830.1 T. thermophila With H59A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S6 D68A Asp68Ala CAA33507.1 A. nidulans With E70A and R72A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S6 D68A Asp68Ala CAA19365.1 S. pombe With E70A, P71A, R72A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D70N Asp70Asn AAB67442.1 (Tub4p) S. cerevisiae With C13S, GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
E70A Glu70Ala CAA33507.1 A. nidulans With D68A and R72A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
E70A Glu70Ala CAA19365.1 S. pombe With D68A, P71A, R72A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H2 P71A Pro71Ala CAA19365.1 S. pombe With D68A, E70A, R72A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H2 G72G* Gly72 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H2 R72A Arg72Ala CAA33507.1 A. nidulans With D68A and E70A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H2 R72A Arg72Ala CAA19365.1 S. pombe With D68A, E70A, P71A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H2 R72A Arg72Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H2 I74T Ile74Thr NP_598785.1 (Tubg1) M. musculus R6564:Tubg1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
H2 K76K* Lys76 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H2 V76V* Val76 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
A81A* Ala81 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
A81A* Ala81 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
G84G* Gly84 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
G84G* Gly84 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
K84A Lys84Ala CAA19365.1 S. pombe With L85A, Y86A, N87A, P88A, E89A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
L85A Leu85Ala CAA19365.1 S. pombe With K84A, Y86A, N87A, P88A, E89A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
Y86A Tyr86Ala CAA19365.1 S. pombe With K84A, L85A, N87A, P88A, E89A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
N87A Asn87Ala CAA19365.1 S. pombe With K84A, L85A, Y86A, P88A, E89A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
N87D Asn87Asp CAA19365.1 S. pombe Suppressor of alp4-1891 mutation Tange et al. 2004. Genetics 167:1095-107 Schizosaccharomyces pombe, fission yeast, fungus
P88A Pro88Ala CAA19365.1 S. pombe With K84A, L85A, Y86A, N87A, E89A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
E89A Glu89Ala CAA19365.1 S. pombe With K84A, L85A, Y86A, N87A, P88A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S7 F92F* Phe92 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
S7 F92F* Phe92 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
S7 Y92C Tyr92Cys ABN11808.1 (G1) H. sapiens Malformations of cortical development Poirier et al. 2013. Nat Genet 45:639-47 Homo sapiens, human
S7 S94S* Ser94 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
S7 S94S* Ser94 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
E95X Glu95stop NP_598789.1 (Tubg2) M. musculus R1378:Tubg2, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
E95X Glu95stop NP_598789.1 (Tubg2) M. musculus R1449:Tubg2, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
A101G Ala101Gly AAB65830.1 T. thermophila Basal body deregulation Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H3 V104A Val104Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H3 E111A Glu111Ala AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H3 K114A Lys114Ala AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
D116A Asp116Ala AAB65830.1 T. thermophila With D117A, cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
E116A Glu116Ala CAA33507.1 A. nidulans With E117A and D120A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H4 D117A Asp117Ala AAB65830.1 T. thermophila With D116A, cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H4 E117A Glu117Ala CAA33507.1 A. nidulans With E116A and D120A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H4 D120A Asp120Ala CAA33507.1 A. nidulans With E116A and E117A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H4 D120A Asp 120Ala CAA19365.1 S. pombe With D123A, R124A, E125A, D127A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H4 D120A Asp120Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H4 D123A Asp123Ala CAA33507.1 A. nidulans With R124A and E125A, weak cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H4 D123A Asp123Ala CAA19365.1 S. pombe With D120A, R124A, E125A, D127A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H4 R124A Arg124Ala CAA33507.1 A. nidulans With D123A and E125A, weak cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H4 R124A Arg124Ala CAA19365.1 S. pombe With D120A, D123A, E125A, D127A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H4 E125A Glu125Ala CAA33507.1 A. nidulans With D123A and R124A, weak cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H4 E125A Glu125Ala CAA19365.1 S. pombe With D120A, D123A, R124A, D127A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H4 E126K Glu126Lys AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H4 D127A Asp127Ala CAA33507.1 A. nidulans With D130A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H4 D127A Asp127Ala CAA19365.1 S. pombe With D120A, D123A, R124A, E125A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S129A Ser129Ala CAA19365.1 S. pombe With D130A, S131A, E133A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D130A Asp130Ala CAA33507.1 A. nidulans With D127A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
D130A Asp130Ala CAA19365.1 S. pombe With S129A, S131A, E133A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S131A Ser131Ala CAA19365.1 S. pombe With S129A, D130A, E133A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S8 E133A Glu133Ala CAA33507.1 A. nidulans Wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S8 E133A Glu133Ala CAA19365.1 S. pombe With S129A, D130A, S131A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S8 S141A Ser141Ala AAB67442.1 (Tub4p) S. cerevisiae With T146A, GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
T146A Thr146Ala AAB67442.1 (Tub4p) S. cerevisiae With Q12G and C13G, GTP binding; with C13S, GTP binding; with S141A, GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
T146A Thr146Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
T146V Thr146Val AAB65830.1 T. thermophila Basal body deregulation Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H5 E155A Glu155Ala CAA33507.1 A. nidulans With R156A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H5 E155A Glu155Ala CAA19365.1 S. pombe With R156A, L157A, D159A, R160A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H5 R156A Arg156Ala CAA33507.1 A. nidulans With E155A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H5 R156A Arg156Ala CAA19365.1 S. pombe With E155A, L157A, D159A, R160A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H5 T156G Thr146Gly AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H5 L157A Leu157Ala CAA19365.1 S. pombe With E155A, R156A, D159A, R160A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H5 D159A Asp159Ala CAA33507.1 A. nidulans With R160A, cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H5 D159A Asp159Ala CAA19365.1 S. pombe With E155A, R156A, L157A, R160A, recessive cold sensitive; with R160A, K163A, K164A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H5 R160A Arg160Ala CAA33507.1 A. nidulans With D159A, cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H5 R160A Arg160Ala CAA19365.1 S. pombe With E155A, R156A, L157A, D159A, recessive cold sensitive; with D159A, K163A, K164A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K163A Lys163Ala CAA33507.1 A. nidulans With K164A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
K163A Lys163Ala CAA19365.1 S. pombe With D159A, R160A, K164A, recessive lethal; with K164A and L165V, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S9 K164A Lys164Ala CAA33507.1 A. nidulans With K163A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S9 K164A Lys164Ala CAA19365.1 S. pombe With D159A, R160A, K164A, recessive lethal; with K163A and L165V, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S9 K164A Lys164Ala AAB65830.1 T. thermophila With K165A, weakly cold sensitive Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S9 K165A Lys165Ala AAB65830.1 T. thermophila With K164A, weakly cold sensitive Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S9 L165V Leu165Val CAA19365.1 S. pombe with K163A and K164A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D176A Asp176Ala CAA19365.1 S. pombe With E177A, S179A, D180A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D176E Asp176Glu NP_598789.1 (Tubg2) M. musculus IGL01419:Tubg2, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
E177A Glu177Ala CAA19365.1 S. pombe With D176A, S179A, D180A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D179A Asp179Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S179A Ser179Ala CAA19365.1 S. pombe With D176A, E177A, D180A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D180A Asp180Ala CAA19365.1 S. pombe With D176A, E177A, S179A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H6 K192A Lys192Ala AAB65830.1 T. thermophila With R193A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H6 R192A Arg192Ala CAA33507.1 A. nidulans With R193A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H6 K193A Lys193Ala CAA19365.1 S. pombe With R194A and L195A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H6 R193A Arg193Ala CAA33507.1 A. nidulans With R192A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H6 R193A Arg193Ala AAB65830.1 T. thermophila With K192A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H6 R194A Arg194Ala CAA19365.1 S. pombe With K193A and R195A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H6 L195A Leu195Ala CAA19365.1 S. pombe With K193A and R194A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S10 D200G Asp200Gly NP_598785.1 (Tubg1) M. musculus R5590:Tubg1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
S10 D205N Asp205Asn AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S10 N206A Asn206Ala AAB67442.1 (Tub4p) S. cerevisiae GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
H7 K211A Lys211Ala AAB65830.1 T. thermophila Weak cold sensitive Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H7 R211A Arg211Ala CAA33507.1 A. nidulans Wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H7 R215A Arg215Ala CAA33507.1 A. nidulans With R216A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R216A Arg216Ala CAA33507.1 A. nidulans With R215A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
I224A Ile224Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
L224A Leu224Ala AAB67442.1 (Tub4p) S. cerevisiae GTP binding; with N206A, GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
N228A Asn228Ala AAB67442.1 (Tub4p) S. cerevisiae GTP binding Gombos et al. 2013. Nat Cell Biol 15:1317-27 Saccharomyces cerevisiae, yeast, fungus
H8 L230T Leu230Thr AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H8 T233A Thr233Ala AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
R243A Arg243Ala CAA33507.1 A. nidulans Weak heat sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R243A Arg243Ala AAB65830.1 T. thermophila Cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
L250L* Leu250 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
L250L* Leu250 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
D251A Asp251Ala AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
R264A Arg264Ala CAA33507.1 A. nidulans With H266A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H266A His266Ala CAA33507.1 A. nidulans With R264A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S11 M270K Met270Lys NP_598785.1 (Tubg1) M. musculus R4090:Tubg1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
S11 I274A Ile274Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
D278A Asp278Ala CAA33507.1 A. nidulans With D281A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
D278A Asp278Ala AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
A280A* Ala280 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
D281A Asp281Ala CAA33507.1 A. nidulans With D278A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
I282I* Ile282 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
I282I* Ile282 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
K284A Lys284Ala CAA33507.1 A. nidulans With R287A and K288A, cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R285A Arg285Ala AAB65830.1 T. thermophila With D291A, cold sensitive Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
R285D Arg285Asp AAB65830.1 T. thermophila With K286D, cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
R285E Arg285Glu AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
R285Q Arg285Gln AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
K286D Lys286Asp AAB65830.1 T. thermophila With R285D, cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
K286E Lys286Glu AAB65830.1 T. thermophila Cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
R286A Arg286Ala CAA19365.1 S. pombe With K287A and T288A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K287A Lys287Ala CAA19365.1 S. pombe With R286A and T288A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K287E Lys287Glu NP_598789.1 (Tubg2) M. musculus R6156:Tubg2, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
R287A Arg287Ala CAA33507.1 A. nidulans With K284A and K288A, cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
K288A Lys288Ala CAA33507.1 A. nidulans With K284A and R287A, cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
T288A Arg286Ala CAA19365.1 S. pombe With R286A and K287A, recessive cold sensitive Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H10 D291A Asp291Ala AAB65830.1 T. thermophila With R285A, cold sensitive Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H10 D292A Asp292Ala CAA19365.1 S. pombe With R295A, R296A, R297A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H10 D293A Asp293Ala CAA33507.1 A. nidulans With D296A and R297A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H10 R294A Arg294Ala AAB65830.1 T. thermophila With R295A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H10 R295A Arg295Ala CAA19365.1 S. pombe With D292A, R296A, R297A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H10 R295A Arg295Ala AAB65830.1 T. thermophila With R294A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H10 R296A Arg296Ala CAA33507.1 A. nidulans With D293A and R297A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H10 R296A Arg296Ala CAA19365.1 S. pombe With D292A, R295A, R297A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H10 P297P* Pro297 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H10 R297A Arg297Ala CAA33507.1 A. nidulans With D293A and R296A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H10 R297A Arg297Ala CAA19365.1 S. pombe With D292A, R295A, R296A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
L301S Leu301Ser CAA19365.1 S. pombe Suppressor of alp4-1891 mutation Tange et al. 2004. Genetics 167:1095-107 Schizosaccharomyces pombe, fission yeast, fungus
K302A Lys302Ala CAA33507.1 A. nidulans With R304A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
P302L Pro302Leu CAA19365.1 S. pombe Suppressor of alp4-1891 mutation, synthetic lethal with Pkl1 Paluh et al. 2000. Mol Biol Cell 11:1225-39, Tange et al. 2004. Genetics 167:1095-107 Schizosaccharomyces pombe, fission yeast, fungus
P302S Pro302Ser CAA19365.1 S. pombe With V307I, suppressor of alp4-1891 mutation Tange et al. 2004. Genetics 167:1095-107 Schizosaccharomyces pombe, fission yeast, fungus
P303P* Pro303 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
R304A Arg304Ala CAA33507.1 A. nidulans With K302A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
V304A Val304Ala AAB65830.1 T. thermophila Slow growth at 30 C Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
V307I Val307Ile CAA19365.1 S. pombe With P302S, suppressor of alp4-1891 mutation Tange et al. 2004. Genetics 167:1095-107 Schizosaccharomyces pombe, fission yeast, fungus
R309A Arg309Ala CAA19365.1 S. pombe With D310A and R311A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D310A Asp310Ala CAA19365.1 S. pombe With D309A and R311A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
R311A Arg311Ala CAA19365.1 S. pombe With R309A and D310A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S12 Y317H Tyr317His NP_598789.1 (Tubg2) M. musculus IGL02693:Tubg2, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
S13 E326A Glu326Ala CAA33507.1 A. nidulans With D328A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
D328A Asp328Ala CAA33507.1 A. nidulans With E326A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 D331A Asp331Ala CAA33507.1 A. nidulans With K334A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 T331P Tyr331Pro ABN11808.1 (G1) H. sapiens Malformations of cortical development Poirier et al. 2013. Nat Genet 45:639-47 Homo sapiens, human
H11 K334A Lys334Ala CAA33507.1 A. nidulans With D331A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 R335A Arg335Ala AAB65830.1 T. thermophila With R337A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H11 R337A Arg337Ala AAB65830.1 T. thermophila With R335A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H11 E338K Glu338Lys AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H11 R338A Arg338Ala CAA33507.1 A. nidulans With R340A, cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 R339A Arg339Ala CAA19365.1 S. pombe With R341A, E342A, R343A, K344A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H11 R340A Arg340Ala CAA33507.1 A. nidulans With R338A, cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 R341A Arg341Ala CAA33507.1 A. nidulans With R342A and R343A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 R341A Arg341Ala CAA19365.1 S. pombe With R339A, E342A, R343A, K344A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H11 E342A Glu342Ala CAA19365.1 S. pombe With R339A, R341A, R343A, K344A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H11 R342A Arg342Ala CAA33507.1 A. nidulans With R341A and R343A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 R343A Arg343Ala CAA33507.1 A. nidulans With R341A and R342A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H11 R343A Arg343Ala CAA19365.1 S. pombe With R339A, R341A, E342A, K344A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K344A Lys344Ala CAA19365.1 S. pombe With R339A, R341A, E342A, R343A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S14 K358A Lys358Ala AAB65830.1 T. thermophila With K359A, cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S14 K359A Lys359Ala AAB65830.1 T. thermophila With K358A, cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
S14 R361A Arg361Ala CAA33507.1 A. nidulans With K362A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S14 K362A Lys362Ala CAA33507.1 A. nidulans With R361A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S14 R362A Arg362Ala CAA19365.1 S. pombe With K363A and S364A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K363A Lys363Ala CAA19365.1 S. pombe With R362A and S364A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
S364A Ser364Ala CAA19365.1 S. pombe With R362 and K363A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H367A His367Ala AAB65830.1 T. thermophila With K368A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
K368A Lys368Ala AAB65830.1 T. thermophila With H367A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H370A His370Ala CAA33507.1 A. nidulans With R371A, weak cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R371A Arg371Ala CAA33507.1 A. nidulans With H370A, weak cold sensitive Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
S15 M377T Met377Thr NP_598785.1 (Tubg1) M. musculus R0599:Tubg1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
H12 L387P Leu387Pro ABN11808.1 (G1) H. sapiens Malformations of cortical development Poirier et al. 2013. Nat Genet 45:639-47 Homo sapiens, human
H12 K388A Lys388Ala CAA33507.1 A. nidulans With R389A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H12 E389A Glu389Ala CAA19365.1 S. pombe With R390A, T391A, R393A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 R389A Arg389Ala CAA33507.1 A. nidulans With K388A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H12 R390A Glu389Ala CAA19365.1 S. pombe With E389A, T391A, R393A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 I391I* Ile391 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 T391A Thr391Ala CAA19365.1 S. pombe With E389A, R390A, R393A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
R393A Arg393Ala CAA19365.1 S. pombe With E389A, R390A, T391A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 R394R* Arg394 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 S394S* Ser394 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 D395A Asp395Ala CAA33507.1 A. nidulans With R396A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H12 N395N* Asn395 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 D396A Asp396Ala CAA19365.1 S. pombe With K397A, R399A, K400A, R401A, E402A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 N396N* Asn396 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 N396N* Asn396 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 K396A Lys396Ala AAB65830.1 T. thermophila With K397A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H12 R396A Arg396Ala CAA33507.1 A. nidulans With D395A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H12 K397A Lys397Ala CAA19365.1 S. pombe With D396A, R399A, K400A, R401A, E402A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 K397A Lys397Ala AAB65830.1 T. thermophila With K396A, wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H12 R398A Arg398Ala CAA33507.1 A. nidulans With K399A and R400A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H12 K399A Lys399Ala CAA33507.1 A. nidulans With R398A and R400A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H12 R399A Arg399Ala CAA19365.1 S. pombe With D396A, K397A, K400A, R401A, E402A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 D400D* Asp400 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 K400A Lys400Ala CAA19365.1 S. pombe With D396A, K397A, R399A, R401A, E402A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 R400A Arg400Ala CAA33507.1 A. nidulans With R398A and K399A, recessive lethal Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H12 N401N* Asn401 ABN11808.1 (G1) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
H12 R401A Arg401Ala CAA19365.1 S. pombe With D396A, K397A, R399A, K400A, E402A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H12 T401T* Thr401 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
E402A Glu402Ala CAA19365.1 S. pombe With D396A, K397A, R399A, K400A, R401A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
Q403Q* Gln403 ABN11809.1 (G2) E. focardii Cold stable microtubules Marziale et al. 2008. FEBS J 275:5367-82, Pucciarelli et al. 2009. Mar Genomics 2:57-66 Euplotes focardii, extremophile, Antarctic ciliate
K405A Lys405Ala AAB65830.1 T. thermophila With K406A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
E406A Glu406Ala CAA19365.1 S. pombe With R409A, K410A, E411A, D412A, cold sensitive diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
K406A Lys406Ala AAB65830.1 T. thermophila With K405A, lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
K408A Lys408Ala CAA33507.1 A. nidulans With K409A and E410A, benomyl dependent Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
K409A Lys409Ala CAA33507.1 A. nidulans With K408A and E410A, benomyl dependent Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R409A Arg409Ala CAA19365.1 S. pombe With E406A, K410A, E411A, D412A, cold sensitive diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
E410A Glu410Ala CAA33507.1 A. nidulans With K408A and K409A, benomyl dependent Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
K410A Lys410Ala CAA19365.1 S. pombe With E406A, R409A, E411A, D412A, cold sensitive diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
E411A Glu411Ala CAA19365.1 S. pombe With E406A, R409A, K410A, D412A, cold sensitive diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D412A Asp412Ala CAA19365.1 S. pombe With E406A, R409A, K410A, E411A, cold sensitive diploid Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D415A Asp415Ala CAA33507.1 A. nidulans With D418A and E419A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
K415A Lys415Ala CAA19365.1 S. pombe With D416A and N417A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D416A Asp416Ala CAA19365.1 S. pombe With K415A and N417A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
N417A Asn417Ala CAA19365.1 S. pombe With K415A and D416A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 D418A Asp418Ala CAA33507.1 A. nidulans With D415A and E419A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 D419A Asp419Ala CAA19365.1 S. pombe With E420A and D422A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 E419A Glu419Ala CAA33507.1 A. nidulans With D415A and F418A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 E420A Glu420Ala CAA19365.1 S. pombe With D419A and D422A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 D421A Asp421Ala CAA33507.1 A. nidulans With E422A and R424A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 D422A Asp422Ala CAA19365.1 S. pombe With D419A and E420A, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 E422A Glu422Ala CAA33507.1 A. nidulans With D421A and R424A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 R424A Arg424Ala CAA33507.1 A. nidulans With D421A and E422A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 R425A Arg425Ala CAA19365.1 S. pombe With E426A and I427A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 E426A Glu426Ala CAA19365.1 S. pombe With R425A and I427A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 I427A Ile427Ala CAA19365.1 S. pombe With R425A and E426A, wild type Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 D429A Asp429Ala CAA33507.1 A. nidulans Wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 Y429A Tyr429Ala AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H13 Y429E Tyr429Glu AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H13 Y429F Tyr429Phe AAB65830.1 T. thermophila Cold sensitive, loss of basal bodies Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H13 Δ429-449 Δ429-449 AAB65830.1 T. thermophila Lethal Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H13 Δ430-449 Δ430-449 AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
H13 D433A Asp433Ala CAA19365.1 S. pombe With E434A and Y435A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 D433X Asp433stop AAF34188.1 H. sapiens No localization to centrosomes Leask and Stearns. 1998. J Biol Chem 273:2661-8 Homo sapiens, human
H13 E433A Glu433Ala CAA33507.1 A. nidulans With E435A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 E434A Glu434Ala CAA19365.1 S. pombe With D433A and Y435A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
H13 E435A Glu433Ala CAA33507.1 A. nidulans With E433A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
H13 Y435A Tyr435Ala CAA19365.1 S. pombe With D433A and E434A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
Y437D Tyr437Asp AAB65830.1 T. thermophila Wild type Shang et al. 2005. J Cell Biol 171:1035-44 Tetrahymena thermophila
E438A Glu438Ala CAA33507.1 A. nidulans With R439A and E440A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R439A Arg439Ala CAA33507.1 A. nidulans With E438A and E440A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
E440A Glu440Ala CAA33507.1 A. nidulans With E438A and R439A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
R440A Arg440Ala CAA19365.1 S. pombe With P441A and D442A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
P441A Pro441Ala CAA19365.1 S. pombe With R440A and D442A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D442A Asp442Ala CAA19365.1 S. pombe With R440A and P441A, cold sensitive diploid, recessive lethal Hendrickson et al. 2001. Mol Biol Cell 12:2469-81 Schizosaccharomyces pombe, fission yeast, fungus
D442X Asp442stop AAF34188.1 H. sapiens No localization to centrosomes Leask and Stearns. 1998. J Biol Chem 273:2661-8 Homo sapiens, human
D443-D447 DAsp443-Asp447 AAB67442.1 (Tub4p) S. cerevisiae Temperature-sensitive growth Vogel and Snyder. 2000. J Cell Sci 113:3871-82 Saccharomyces cerevisiae, yeast, fungus
D444A Asp444Ala CAA33507.1 A. nidulans With D446A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
Y445D Tyr445Asp AAB67442.1 (Tub4p) S. cerevisiae Anaphase arrest, benomyl resistant Vogel et al. 2001. Dev Cell 1:621-31 Saccharomyces cerevisiae, yeast, fungus
Y445X Tyr445Stop AAB67442.1 (Tub4p) S. cerevisiae Temperature-sensitive growth Vogel and Snyder. 2000. J Cell Sci 113:3871-82 Saccharomyces cerevisiae, yeast, fungus
D446A Asp446Ala CAA33507.1 A. nidulans With D444A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
K449A Lys449Ala CAA33507.1 A. nidulans With D450A and E451A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
V449X Val449Stop AAB67442.1 (Tub4p) S. cerevisiae Temperature-sensitive growth Vogel and Snyder. 2000. J Cell Sci 113:3871-82 Saccharomyces cerevisiae, yeast, fungus
D450A Asp450Ala CAA33507.1 A. nidulans With K449A and E451A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus
E451A Glu451Ala CAA33507.1 A. nidulans With K449A and D450A, wild type Jung et al. 2001. Mol Biol Cell 12:2119-36 Aspergillus nidulans, fungus

Delta/Zeta Tubulin Mutations

XRD AA1 AA3 Tubulin Gene (Isotype) Organism Phenotype Reference Keywords Notes
S30F Ser30Phe NP_001185974.1 (Tubd1) M. musculus R4436:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
S30F Ser30Phe NP_001185974.1 (Tubd1) M. musculus R4436:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
Q82X Gln82Stop NP_001185974.1 (Tubd1) M. musculus R0039:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
G107S Gly107Ser NP_001185974.1 (Tubd1) M. musculus Z1088:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
G107S Gly107Ser NP_001185974.1 (Tubd1) M. musculus R4153:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
V110L Val110Leu NP_001185974.1 (Tubd1) M. musculus R4890:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
G178C Gly178Cys NP_001185974.1 (Tubd1) M. musculus IGL03088:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
K211M Lys211Met NP_001185974.1 (Tubd1) M. musculus Z1088:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
I219N Ile219Asn NP_001185974.1 (Tubd1) M. musculus R2305:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
L256P Leu256Pro NP_001185974.1 (Tubd1) M. musculus R4773:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
Q279K Gln79Lys NP_001185974.1 (Tubd1) M. musculus R0427:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
V305G Val305Gly NP_001185974.1 (Tubd1) M. musculus R0482:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
M311L Met311Leu NP_001185974.1 (Tubd1) M. musculus R6192:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
L326P Leu326Pro NP_001185974.1 (Tubd1) M. musculus R4990:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
T371I Thr371Ile NP_001185974.1 (Tubd1) M. musculus R5004:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
V374F Val374Phe NP_001185974.1 (Tubd1) M. musculus IGL00159:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
Y457H Tyr457His NP_001185974.1 (Tubd1) M. musculus R4908:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
M462T Met462Thr NP_001185974.1 (Tubd1) M. musculus R4793:Tubd1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
Y470C Tyr470Cys CAB99490.1 (Zeta tubulin) P. tetraurelia Thermosensitive recessive mutation, causes reduction in number of basal bodies, reduced cell length, modified shape and delocalization of gamma-tubulin Ruiz et al. 2000. Current Biol 10:1451-4. Paramecium tetraurelia, ciliate, SM19, SM 19, eta tubulin

Epsilon Tubulin Mutations

XRD AA1 AA3 Tubulin Gene (Isotype) Organism Phenotype Reference Keywords Notes
V7I Val7Ile NP_082282.1 (Tube1) M. musculus R6331:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
V7I Val7Ile NP_082282.1 (Tube1) M. musculus R6331:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
G10X Gly10stop NP_082282.1 (Tube1) M. musculus R1251:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
V80I Val80Ile NP_082282.1 (Tube1) M. musculus IGL02437:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
S144G Ser163Gly AAM23012.1 C. reinhardtii Reversion of loss of flagella/immotile phenotype induced by I163N mutation (second mutation) Esparza et al. 2013. PLoS One 8 Chlamydomonas reinhardtii, algae
M147V Met147Val NP_082282.1 (Tube1) M. musculus R4646:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
I163N Iso163Asn AAM23012.1 C. reinhardtii Lacks flagella/motility Esparza et al. 2013. PLoS One 8 Chlamydomonas reinhardtii, algae
N163S Asn163Ser AAM23012.1 C. reinhardtii Reversion of loss of flagella/immotile phenotype induced by I163N mutation Esparza et al. 2013. PLoS One 8 Chlamydomonas reinhardtii, algae
D210E Asp210Glu NP_082282.1 (Tube1) M. musculus R5267:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
D216G Asp216Gly NP_082282.1 (Tube1) M. musculus R1607:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
N243D Asn243Asp NP_082282.1 (Tube1) M. musculus R4840:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
L267P Leu267Pro NP_082282.1 (Tube1) M. musculus R2367:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
M281K Met281Lys NP_082282.1 (Tube1) M. musculus R0145:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse
H331Q His331Gln NP_082282.1 (Tube1) M. musculus R2138:Tube1, viable heterozygote, ENU mutatgenesis Mutagenetix Mus musculus, mouse